Label-free microscopy offers the option to gain chemical as well as morphological information without the need of staining the sample or genetically labelling them. In a multimodal microscopy approach, multiple nonlinear microscopy techniques are employed to image biological samples.
The multimodal microscope will feature a few complimentary imaging techniques. Coherent Raman microscopy makes use of two laser pulses. If the frequency separation of these pulses matches a vibrational resonance of an organic molecule this leads a shifting of photons from one pulse to the other. This technique offers recording specific molecular fingerprints, providing highest chemical sensitivity on a single-cell level. In addition, the laser pulses generate other nonlinear signals including second harmonic generation or multiphoton auto-fluorescence, providing additional morphological information, which can be combined with the fingerprints.
Our team at the Laboratory for Attosecond Physics (LAP) at the Ludwig-Maximilians-Universität Munich and Max Planck Institute of Quantum Optics in Garching is currently looking for a motivated MSc or PhD student to join our development of a multimodel microscope for medical applications, including in particular the detection of early-stage cancer.
Experience in nonlinear optics and laser physics or microscopy can be beneficial but are not required.
For more information, please visit our homepage at: http://www.attosecondimaging.de
If you are interested in joining our team, please contact: Prof. Dr. Matthias Kling,
28th November 2017Multimodal microscope image of cancer cells: Coherent Raman signal of lipids (red) and two photon autofluorescence (green)